MUIH Microbial Analysis Experiment Internship Part II

The second part of this post covers the first phase of the actual experiment.  If you missed the first part of this post, you can find it here.  Technically, this is no longer an internship because I didn’t do it for college or work credit.  After all the research and planning for the experiment, I wanted to be part of it.  I was excited about the prospect of participating in a study between two universities, with the possibility of the findings being published.

We were testing the theory that a bunsen burner could create a sterile field. The reason this would be cool is because many product makers are small businesses that cannot afford to rent lab space for product creation.  The execution of the experiment required learning and using aseptic lab techniques (limiting variables), meticulously documenting each step and then counting colonies.

 

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The bunsen burner was turned on for five minutes to create the sterile field.  Measurements on the table indicate the distance of each TSA plate (a.k.a. a petri dish to most) to the burner.  After 5 minutes the lids were removed to expose them to the microbes in the air.
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After the predetermined amount of time the lids were replaced and the dishes were wrapped in lab film to keep them secure.
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Close up of the dishes before the incubation period.
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Here is a couple of days in.

 

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This is after 7 days of incubation. Look at all of those organisms!

The study has not been published yet so I will not discuss the results.  Needless to say, my personal results were… gross.  It was really awesome learning about aseptic lab techniques and getting to participate in this study.

Part III of this post will be the final part and will cover the second, and last, experiment conducted as part of this study.

 

 

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Author: thebotanicalengineer

Herbalist, aromatherapist, product designer, and plant scientist.

2 thoughts on “MUIH Microbial Analysis Experiment Internship Part II”

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